Simple optimization procedure for objective lens correction collar setting

J. Microsc., Vol. 217, Pt. 3, March 2005, 184-187. M. Schwertner, M.J. Booth & T. Wilson.

We present a new method for setting a coverglass correction

collar on an objective lens. Axial scans across the interface

between the specimen volume and the slide are used together

with a quantitative function of merit to determine the

optimum setting of the correction collar. The method, which

simplifies the adjustment for the user and reduces photobleaching,

was implemented within the software environment

of a scanning microscope system.

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