Real time 3D fluorescence microscopy by two beam interference illumination

OPT COMMUN 153 (1-3): 1-4 JUL 15 1998 Neil MAA, Juskaitis R, Wilson T

We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope byinterfering two beams on an object so as to illuminate it with a single spatial frequency fringe pattern. Images taken at threespatial positions of the fringe pattern are processed in real time to produce optically sectioned images which are substantiallysimilar to those obtained with confocal microscopes.

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