An optical technique for refocusing in microscopy

Optics Communication Volume 281, Issue 4, 15 Feb 2008, 880-887. doi:10.1016/j.optcom.2007.10.007 E. J. Botcherby, R. Juskaitis, M. J. Booth & T. Wilson

We describe the theory of a new method of optical refocusing that is particularly relevant for confocal and multiphoton microscopy systems. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free refocusing can be achieved over an axial scan range of 70 lm for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method enables high axial scan speeds without mechanical interference between the objective lens and the specimen.

null som_2008_2.PDF — PDF document, 243Kb