Adaptive harmonic generation microscopy of mammalian embryos

Optics Letters, Volume 34, Issue 20, Oct 2009, 2495-2497. doi:10.1364/OL.34.003154 A. Jesacher, A. Thayil, K. Grieve, D. Débarre, T. Watanabe, T. Wilson, S. Srinivas & M. Booth

Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

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